ALDH2/ADH1B Genotyping Kit


·Provide nitroglycerin medication guidance and healthy alcohol consumption advice

·Assist doctors in selecting the appropriate nitroglycerin dose or changing to other medications to avoid drug resistance or adverse effects

·Test once, benefit lifetime

Nitroglycerin tolerance

Nitroglycerin, which treats impaired cardiac function through vasodilation as it is converted to nitric oxide, is used worldwide for patients with various ischemic and congestive cardiac diseases, including angina pectoris.

However, its efficiency differs widely among individuals. Some reports show that nitroglycerin has no effect in more than 25% of Asian people because of the gene mutation.

Nitroglycerin tolerance is a result of inactivation of aldehyde dehydrogenase 2 (ALDH2). ALDH2 is the key enzyme for the biotransformation from nitroglycerin into effective metabolite nitric oxide (NO). A research, conducted by Rui jin Hospital of Shanghai Jiao Tong University and School of Life Science of Fudan University, found that when ALDH2 rs671 mutation happens (Glu504Lys), the enzyme activity of ALDH2 decreases more than 10 times, which weakens its ability of releasing NO[1]. Therefore, ALDH2 genotyping is essential before accepting nitroglycerin treatment.

ALDH2 and ADH1B: Key genes for alcohol metabolism

The ethanol detoxifying pathway in humans occurs mainly in the liver and is carried out by two enzymatic steps.The primary enzymes involved in alcohol metabolism are alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH).

ADH1B is one of most important ADH genes, and SNPs in ADH1B will result in the production of enzymes with different activities. The presence of ADH1B *2 allele would be associated with an increased oxidizing capacity[2].

Ethanol metabolism occurs in two steps: ethanol is metabolized quickly by alcohol dehydrogenase (ADH) to generate acetaldehyde. Acetaldehyde is then metabolized by the mitochondrial aldehyde dehydrogenase 2 (ALDH2) to acetate.

ALDH2 is better known for its critical role in ethanol metabolism, which is probably the only ALDH enzyme that contributes significantly to acetaldehyde metabolism. The ALDH2*2 carriers have a lower ALDH2 enzymatic activity and this deficiency is manifested by the characteristic facial flushing, headaches, nausea, dizziness, and cardiac palpitations after consumption of alcoholic beverages[3].

Ethanol metabolism occurs in two steps: ethanol is metabolized quickly by alcohol dehydrogenase (ADH) to generate acetaldehyde. Acetaldehyde is then metabolized by the mitochondrial aldehyde dehydrogenase 2 (ALDH2) to acetate.

Nitroglycerin medication and health drinking guide based on ALDH2/ADH1B genotyping

Product introduction

Applying Advanced Fragment Analysis (AFA) technology, the kit provides fast, accurate and effective genotyping.It simultaneously amplifies 2 SNP loci of ALDH2 and ADH1B in one tube and separates the different size of fragments by capillary electrophoresis. It is used for nitroglycerin medication guidance and healthy alcohol consumption advice.


· High accuracy:100% consistency with the Sanger sequencing results (>1400 samples)

· Sample compatibility: suitable for either blood or buccal swab

· Time saving: from sample to answer within 4 hours, and hands-on time nearly 0.5 hours

· User friendly:8-tube strip format, add enzyme and sample to start; automated report

· Monitor every single reaction:build in internal reaction control for monitoring PCR process

· Free of carry-over contamination: build in dUTP-UNG system


Example electropherogram of ALDH2/ADH1B genotyping

Order Information

SureX® ALDH2/ADH1B Genotyping Kit (CE-IVD)24 tests/kit1060073ABI 3130/3130xl/3500 Dx/3500xL Dx


[1] Yifeng Li, Dandan Zhang, et al. Mitochondrial aldehyde dehydrogenase-2 (ALDH2) Glu504Lys polymorphism contributes to the variation in efficacy of sublingual nitroglycerin. J Clin Invest. 2006 Feb; 116(2):506-11.

[2] Peng GS, Yin SJ. Effect of the allelic variants of aldehyde dehydrogenase ALDH2*2 and alcohol dehydrogenase ADH1B*2 on blood acetaldehyde concentrations. Hum Genomics. 2009 Jan; 3(2):121-7.

[3] Quillen EE, Chen XD et al. ALDH2 is associated to alcohol dependence and is the major genetic determinant of "daily maximum drinks" in a GWAS study of an isolated rural Chinese sample. Am J Med Genet B Neuropsychiatr Genet. 2014 Mar; 165B(2).

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