13 Respiratory Pathogen Multiplex Kit


·Comprehensive: simultaneous identification of 11 most common respiratory viruses, chlamydia and Mycoplasma pneumonia

·Convenient: single-tube one-step multiplex RT-PCR

·Precise: triple controls and anti-contamination system


·Respiratory tract infection is one of the most common diseases, accounting for 10% of hospitalized patients and several million deaths around the world annually.

·An adolescent or an adult might be infected with respiratory tract pathogens 2 to 4 times each year, while this number may double for a child.

·About 90% of acute upper respiratory tract infections and most of lower respiratory tract infections are caused by respiratory tract pathogens other than bacteria. The common pathogens include but are not limited to human respiratory syncytial virus, human adenovirus, influenza virus and mycoplasma, infections of which result in similar symptoms: fever, cough, weakness, pneumonia or non-leukocytosis.

·Timely identification of respiratory tract pathogens can guide specific treatment, reduce hospital costs, and minimize the excessive use of antibiotics.

Clinic significance

Sample type

·Oropharyngeal swab: flocking swabs and cryogenic vials

·Sputum: sputum aspirators and sterile sputum cups

Sample delivery and storage

An example electropherogram of positive control


· Multiplex: Adaptable to detection of multiple pathogens simultaneously

· Single-tube: Perform cDNA synthesis and multiplex PCR in one tube

· Comprehensive: Identify 11 most common respiratory viruses, chlamydia and Mycoplasma pneumonia

· Sensitive: High sensitivity similar to qPCR

· Efficient: Perform the whole assay within 4 hrs; Hands-on time as short as 0.5 hr; Up to 192 samples within 8 hrs

·  Accurate:

   1) Three controls monitor sample quality and the whole assay, reducing risk of false negatives

   2) No cross-reaction with other pathogens

   3) Efficient anti-contamination system, reducing risk of false positives

· Objective: Normalize signaling on CE platforms by standard mix; Results output by software.

· Compatible: Work well on oropharyngeal swabs and sputum; Perform PCR on common thermal cyclers and

   CE platforms (GeXP/CEQ or ABI3130/3500).

· Economic: Much cheaper than qPCR (for 13 pathogens detection) and liquid chip tests.

Targets List

Assay workflow

Order Information




    Compatible platform

SureX® 13 Respiratory Pathogen Multiplex Kit

50 tests/kit



[1] Zhang H, Cheng H, Wang Q, et al. An advanced fragment analysis-based individualized subtype classification of pediatric acute lymphoblastic leukemia. Sci Rep. 2015. DOI: 10.1038/srep12435.

[2] WANG Q, LIN G, GAO J, et al. Detection of human rhinovirus in cerebrospinal fluid of children with viral encephalitis by using GeXP genetic analysis system. Laboratory Medicine and Clinic. 2015 (13). DOI: 10.3969/j.issn.1672-9455.2015.13.005.

[3] Harris AM, Hicks LA, and Qaseem A. Appropriate Antibiotic Use for Acute Respiratory Tract Infection in Adults: Advice for High-Value Care From the American College of Physicians and the Centers for Disease Control and Prevention. Ann Intern Med. 2016. 164(6):425-434.

[4] Dignan FL, Clark A, Aitken C, et al. BCSH/BSBMT/UK clinical virology network guideline: diagnosis and management of common respiratory viral infections in patients undergoing treatment for haematological malignancies or stem cell transplantation.Br J Haematol. 2016, 173(3):380-93.

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